Description
This Human Podocalyxin-Like Protein 2 (Endoglycan) ELISA Kit is intended for quantitative detection of human Endoglycan/PODXL2 in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.
This human Endoglycan/PODXL2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Endoglycan/PODXL2 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: G33-T500) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Endoglycan/PODXL2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. Expression system for standard: Podocalyxin like 2 is a protein that in humans is encoded by the PODXL2 gene. It is mapped to 3q21.3. This gene is a member of the CD34 family of cell surface transmembrane proteins, which are characterized by an N-terminal extracellular mucin domain, globular and stalk domains, a single pass transmembrane region, and a charged cytoplasmic tail. The encoded protein is a ligand for vascular selectins